Product Description：
Convoy Platinum DNA In Vitro Tranfection Reagent is a powerful transfection reagent that ensures effective and reproducible transfection with invisible toxicity. ConvoyPlatinum is formulated by unique chemistry (covalently cross-linking cationic lipids with polymer), giving rise to exceptional transfection efficiency with distinguishable features in comparison of other types of reagents. ConvoyPlatinum was shown to deliver genes to various established cell lines as well as primary cells including HEK293, 293T, 293E, CHO, COS1, HeLa, NIH 3T3, insect cell lines (Sf9 and Sf21) and a variety of other eucaryotic cell lines. ConvoyPlatinum reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.
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Applications :
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Deliver DNA/RNA into mammalian, insect cells |
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Greatly enhance (up to 200 times higher efficiency) virus (e.g., replication-deficient Adenovirus) infection of hard-to-transfect cells |
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Features :
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No cytotoxicity for most of tumor cell lines and primary cells |
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Exceptional transfection efficiency of a broad range of cell types |
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Efficient transfection with or without serum |
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Simple, robust transfection procedure |
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Effectively transfects both adherent and suspension cell cultures |
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Exceptional high titers of virus production |
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Equally good for very long DNAs (up to 25 kb) |
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Equally good for single or multiple DNA transfections |
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Exceptional high levels of recombinant protein production |
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Top choice for hard-to-transfect cells |
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Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.
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Broad Transfection Spectrum for Mammalian Cell Types
Cell Lines |
Transfection Efficiency
(% GFP) |
Cell Lines |
Transfection Efficiency
(% GFP) |
3LL |
51% |
K562 |
22% |
B16-F10 |
72% |
L929 |
52% |
BAEC |
52% |
MCF-7 |
51% |
BHK-21 |
86% |
MDCK |
52% |
Ca Ski |
78% |
Neuro 2A |
72% |
CaCo2 |
32% |
NIH 3T3 |
88% |
CHO |
83% |
PC12 |
34% |
HCS-2/8 |
42% |
SH-SY5Y |
14% |
HEK-293 |
80-90% |
SiHa |
62% |
HeLa |
81% |
SKOV3 |
52% |
HLMEC |
50-65% |
HUVEC |
51% |
H-MVEC |
49% |
IGROV1 |
21% |
Huh-7D12 |
22% |
Jurkat |
5%-15% |
ATT20 |
38% |
6CSFMEo |
73% |
SK-N-SH |
20% |
WEHI 231 |
25% |
McArdle 7777 |
60% |
SAOS-2 |
52% |
Hep3D |
78% |
SN56 |
69% |
SHEP |
71% |
MC3T3-E1 |
80% |
MDA-MB-231 |
38% |
BT474 |
46% |
3T3 -442A |
10% |
C 2C 12 |
46% |
COS-7 |
60-70% |
Primary mouse keratinocytes |
40-50% |
CV-1 |
53% |
Primary human pre-adipocytes |
40-50% |
D 407 |
62% |
Primary human skin fibroblasts |
25% |
DHD Pro.b |
51% |
Primary mouse embryonic fibroblast |
20% |
LS180 |
46% |
Primary melanocyte |
46% |
A549 |
78% |
Hela-S3 |
78% |
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Primary rat aortic smooth muscle cells were prepared and transfected with a nuclear targeted GFP cDNA by ConvoyPlatinum(left panel) and Lipofectamine 2000 (L2000, right panel) respectively.
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Three DNAs co-transfected with ConvoyPlatinum In Vitro DNA Transfection Reagent (left panel) in comparison of LipoFectamine 2000? (L2000, right panel). 100 microliter virus supernatant from 293F cells were used to counter-infected 293F cells which was then passed FACS. The numbers at the upper right corner indicate the percentage of transduced cells. The titers of lentivirus generated with ConvoyPlatinum and L2000 were quantified to be 7.9x106 and 3.1x106 tu/ml respectively.
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Comparison of cytotoxicity of ConvoyPlatinum DNA In Vitro Transfection Reagent with L2000 on primary murine skin fibroblast. The primary murine fibroblast was incubated with the indicated transfection reagents/pEGFP-C1 (DNA) complexes above for 4 hours in serum-free DMEM High Glucose medium followed by replacement of complete serum-containing medium. The cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging 24 hours post transfection.
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