Description：
3:1 Agarose is a molecular biology grade, standard melting point, unique mixture of agarose formulated to provide high resolution of small nucleic acids and PCR products ?1,000 bp. Its optimized gel strength results in easy-to-handle gels, enhancing the convenience of gel processing and blotting. Deisgned for analytical electrophoresis. Manufactured using innovative Organic Solvent Free Manufacturing process that is greener and more environment friendly.
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Application：Ideal for PCR product separation and blotting and analytical electrophroesis of DNA and RNA fragments ?1,000 bp.
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Storage ：store at room temperature. Keep dry.
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Size : 1 g, 5 g, 25 g, 100 g, 500 g, 1 kg, in bulk
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Features：
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Unique mixture of agarose formulated to
provide high resolution of nucleic acids
?1,000 bp |
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Greener agarose choice |
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Ultra high resolution with high clarity
and low background |
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Optimized gel strength for easy?tohandle
gels |
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No detectable nuclease and protease |
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Specifications：
| Product Name |
3:1 Agarose |
| CAS |
N/A |
| Appearance |
White to off-white powder |
| Solubility |
clear colorless solution at 2g in 100ml water |
| Moisture |
<10% |
| Ash |
< 0.5% |
| Gel strength |
> 650 g /cm2 (1.5% Gel) |
| EEO(-Mr) |
< 0.1 |
| Sulfate |
< 0.1% |
| Gelling point |
36℃（1.5% Gel） |
| Melting point |
< 80℃（1.5% Gel） |
| DNase |
NONE |
| RNase |
NONE |
| Protease |
NONE |
| Endonuclease/ligase inhibitory factor |
NONE |
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Gel Preparation Protocol
1. To make gels with agarose concentration less than 2%:
| (1) |
Use a flask that is 2 to 4 times the volume of the solution being prepared. |
| (2) |
Add the correct amount of dry agarose to a measured quantity of electrophoresis buffer. |
| (3) |
If use a boiling water bath：
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To melt the agarose, simply by heating the slurry in a boiling water bath，bring the solution to a boil and allow it to boil for 5-10 minutes stirring continuously, until the agarose dissolves completely. |
| If use a microwave oven： |
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To melt the agarose in solutions of less than 2%, heat the slurry in microwave oven on high power setting until it starts to boil. |
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Allow the solution to boil for 1 min or until the solution is clear and all particles are dissolved. |
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Remove the flask from the microwave oven, and gently swirl to mix the agarose solution.
Use caution when handling as solution may be extremely heated.
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| (4) |
Cool the solution to approx. 60°C before pouring. |
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2.To make gels with agarose concentration greater than 2%:
| (1) |
Use a flask that is 2 to 4 times the volume of the solution being prepared. |
| (2) |
Add the correct amount of dry agarose to a measured quantity of electrophoresis buffer. |
| (3) |
Heat the slurry in a microwave oven on a medium power setting until it starts to boil. |
| (4) |
Remove the flask from the oven and gently swirl to resuspend the gel particles. |
| (5) |
Reheat the solution on a medium power setting until it starts to boil again. |
| (6) |
Afterwards, remove the flask from the microwave and gently swirl.
If the agarose did not completely dissolve, reheat the solution again.
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| (7) |
Cool to approx. 70°C before pouring. |
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Separation of DNA in agarose
Size range (bp) |
500 ─ 1,000 |
100 ─ 500 |
10 ─ 100 |
Agarose in gel (%) 1 ╳ TAE Buffer |
3.0 |
4.0 |
6.0 |
Agarose in gel (%) 1 ╳ TBE Buffer |
2.0 |
3.0 |
5.0 |
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