G418 Sulfate (Catalog No. : AV418)

Size: 5g

For research only. Not for human or drug use.

G418 sulfate is an aminoglycosidic antibiotic, it acts by interfering with protein synthesis and is usually used as an agent for selection of cultured cells expressing a gene that confers resistance to G418. It is toxic to both prokaryotic and eukaryotic cells. It is commonly used in molecular biology studies.

Specification

Formula: C₂₀H₄₀N₄O₁₀ ·2H₂SO₄
Formula weight: 692.71
Appearance: white powder
Specific Rotation: +104^o ~ +121^o
Moisture: 10%
Absorbance: <0.015 (280nm,1mg/ml), <0.1 (570nm, 100mg/ml)
Potency: 735 ug/mg

Directions for use

The optimal concentration of G418 required to select and maintain cells must be determined experimentally for any given set of growth conditions and should be re-established when those conditions are altered. In general, concentrations of 400 to 1100 ug/ml have been used for selection of mammalian cells, and concentrations of 200 to 400 ug/ml are used for maintenance. G418 may be dissolved or diluted in the culture medium.

Storage conditions

Dry powder should be stored at room temperature, and solution at -20℃.