Description：
HR high resolution agarose is medium gelling point agarose, suitable for the efficient separation of small DNA fragments between 20 and 800 bp in length. It is suitable for the analysis of AFLP's (Amplified Fragment Length Polymorphisms), STR's(Short Tandem Repeats) and tetranucleotide repeats. The low melting temperature of HR agarose makes it an excellent medium for analytical and preparative electrophoresis.
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Application：Ideal for recovery of DNA and RNA. Analytical and preparative electrophoresis of DNA and RNA of 20 to 800 bp in length.
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Storage ：store at room temperature. Keep dry.
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Size : 1 g, 5 g, 25 g, 100 g, 500 g, 1 kg, in bulk
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Features：
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Ideal for small fragment nucleic acid analysis in the range of 20 - 800 bp |
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Greener agarose choice |
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Ultra high resolution with high clarity and low background |
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Intermediate melting point |
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Specifications：
| Product Name |
HR Agarose, PCR Grade |
| CAS |
39346-81-1 |
| Appearance |
White to off-white powder |
| EEO(-Mr) |
< 0.1 |
| Gelling point |
< 33℃（1.5% Gel） |
| Melting point |
< 70℃（1.5% Gel） |
| Solubility |
clear colorless solution at 2g plus 100ml water |
| Moisture |
<10% |
| Gel strength |
> 750 g/cm2 （1.5% Gel） |
| Sulfate |
< 0.1% |
| Ash |
< 0.5% |
| DNase |
NONE |
| RNase |
NONE |
| Protease |
NONE |
| Endonuclease/ligase inhibitory factor |
NONE |
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Gel Preparation Protocol
1. To make gels with agarose concentration less than 2%:
| (1) |
Use a flask that is 2 to 4 times the volume of the solution being prepared. |
| (2) |
Add the correct amount of dry agarose to a measured quantity of electrophoresis buffer. |
| (3) |
If use a boiling water bath：
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To melt the agarose, simply by heating the slurry in a boiling water bath，bring the solution to a boil and allow it to boil for 5-10 minutes stirring continuously, until the agarose dissolves completely. |
| If use a microwave oven： |
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To melt the agarose in solutions of less than 2%, heat the slurry in microwave oven on high power setting until it starts to boil. |
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Allow the solution to boil for 1 min or until the solution is clear and all particles are dissolved. |
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Remove the flask from the microwave oven, and gently swirl to mix the agarose solution.
Use caution when handling as solution may be extremely heated.
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| (4) |
Cool the solution to approx. 60°C before pouring. |
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2.To make gels with agarose concentration greater than 2%:
| (1) |
Use a flask that is 2 to 4 times the volume of the solution being prepared. |
| (2) |
Add the correct amount of dry agarose to a measured quantity of electrophoresis buffer. |
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Heat the slurry in a microwave oven on a medium power setting until it starts to boil. |
| (4) |
Remove the flask from the oven and gently swirl to resuspend the gel particles. |
| (5) |
Reheat the solution on a medium power setting until it starts to boil again. |
| (6) |
Afterwards, remove the flask from the microwave and gently swirl.
If the agarose did not completely dissolve, reheat the solution again.
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| (7) |
Cool to approx. 70°C before pouring. |
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Separation of DNA in agarose
Agarose in gel (%) |
2.0 |
3.0 |
4.0 |
5.0 |
DNA Size range (bp) |
500 ─ 2,000 |
100 ─ 1,200 |
50 ─ 500 |
20 ─ 250 |
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