96-Well Gradient Quantitative PCR Machine | avansbio - Exceeding Expectations as a Cost Effective Provider

Introduction
With the FRTTC-96-G, we bring gradient real-time quantitative PCR instrumentation to your laboratory with a high-performance, full-featured, and low-cost system. The instrument combines precise thermal control, broad wavelength detection, and powerful software that is easy to learn and use. Most importantly, the FRTTC-96-G system delivers outstanding results with all QRCR chemistries.

Innovation Design

Nova optics system

Unique optical fiber technology

Exciting the fluorophore and collecting the emission signal from each well independently and simultaneously.

Signal Collection Technology

Collecting signal from the side of the tube bottom that prevents the reflection of the excitation light and broadens the type of compatible tube.

Four-color filter

Detecting a various of fluorophores in single tube, including FAM, SYBR Green I, TET, HEX,VIC, TAMRA, ROX, Texas Red, Cy3 and Cy5.

Thermal cycling

6 independent temperature control modules.

Gradient temperature range over 20°C in 12 columns.

Powerful software

Powerful data analysis

Absolute Quantification, Relative Quantification, Multiple Quantification, Mutation Analysis, SNP Analysis, Allelic Analysis, △△Ct Analysis, Dissociation Curve, Standard Sample Curve and Melting Curve Detection will be provided real time, and all data can be re-analyzed. PCR efficiency calculation and calibration well by well, SYBR Green I calibration.

Powerful data management

Wide range of personal preferences may be programmed by different operators.

User friendly interface

Easily sets up plate parameters and edits the format of your report, and all reports output could be in Excel format.

Features

1.	High-Resolution Melting(HRM). A technique, based on high resolution (0.1oC increment) melting of amplified products in the presence specific fluorescent dsDNA-binding dye, used for mutation scanning and genotyping without labeled probes.
2.	Short running time. For 5-30ul, 40 cycles can be finished within 40 mins.
3.	Short readout time. 96 well plate can be detected within 1 seconds.
4.	Long lifetime of halogen lamp.
5.	Broad dynamic range. Up to 10 orders of magnitude in starting copy number.
6.	High sensitivity. To detect single copy of the initial template.
7.	Peltier-based thermal cycle system enhances the precision of temperature.
8.	Powerful, versatile software including plate set-up user interface and automated analysis tools make data processing simple and straightforward.

Specifications

Thermal Cycle System
Thermal Mode	Peltier-based TE module
Thermal Gradient	12 Columns
Multiple Temperature Control	6 areas
Heating/Cooling Rate	>3 ℃ /S
Precision of Temperature Control Homogeneity of Temperature	±0.1 ℃ ± 0.25 ℃

Optical system
Excitation Light Source	Halogen Lamp (>2000hours)
Wavelength of Excitation Light	400-700 nm
Wavelength of Detection	450-750nm
Detection Gateway	4 filter provided
Detection System	Cooled CCD
Mode of Detection	Real Time Dynamic Detecting and Displaying. Four Colors can be detected simultaneously.

Detection Performance
Sensitivity of Detection	<10 copies of Target Gene Can be Detected at 99.7% Confidence
Time of Readout	96 well plate can be detected within 3 seconds.
Time of Sample analysis	96 well plate can be analyzed within seconds.
Order of Magnitude	10 orders, 10⁰-10¹⁰

Fluorescent System
Fluorescence Report Dyes	FAMTM/SYBR-Green I, VICTM/JOETM,TAMRATM, ROX, Cy3, Cy5, and Texas Red etc.
Fluorescent Quantitative Methods	Taqman Probe, SYBR Green I Dye, Taqman MGB Probe , and Molecular Beacon etc.

Sample
Sample Format	0.2ml single tube, 8-strip tube, and 96-well plate
Sample Capacity	96 samples per run
Sample Volume	10-100ul
Reaction Vessel	0.2ml
Running Time	5-30ul, 40cycles can be finished within 40mins

Email : info@avansbio.com