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Avans Bio Color Prestained Protein Marker
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Description
The Color Prestained Protein Marker contains 11 proteins that
resolve into sharp, tight bands in the range of 10-175 kDa. The Color Prestained Protein Marker allows you to monitor molecular weight
separation during electrophoresis, estimate molecular weights of
proteins of interest, and evaluate western transfer efficiency.
Features
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Broad range: 10-175 kDa
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Ready-to-use: supplied in a loading buffer for direct loading on gels
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Easy to identify: includes the ~10, ~40 and ~90 kDa reference
bands coupled with an blue dye
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Sharp bands
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Applications
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Monitoring of protein migration during SDS-polyacrylamide gel
electrophoresis.
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Monitoring of protein transfer onto membranes during Western
blotting.
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Sizing of proteins on SDS-polyacrylamide gels and Western blots.
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Quality Control : Tested in SDS-polyacrylamide gel electrophoresis and Western blotting.
Storage :
Stable at +4°C for 3 months
Store at -20°C for 24 months
Recommendations for Loading
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Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil.
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Mix thoroughly to ensure the solution is homogeneous.
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Load the following volumes of the ladder on SDS-polyacrylamide gel:
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- 5 μl per well for mini-gels, 2.5 μl per well for blots
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- 10 μl per well for large gels, 5 μl per well for blots
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