Trio-10 prestained protein marker


AvansBio Trio-10 Prestained Protein Marker is a three-color protein standard with 10 prestained proteins covering a wide range molecular weights for 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Trisglycine buffer). Trio-10 Prestained Protein Marker is designed for monitoring protein separated during SDSpolyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon or nitrocellulose) and for approximate sizing of proteins. The Marker is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.


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Broad range: 10-180 kDa
Ready-to-use: Supplied in a loading buffer for direct loading on gels
Easy to identify: One green and one red band at 25 kDa and 75 kDa respectively
Sharp bands


3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
2 ~ 3 μl per well for general Western transferring.
Apply more for thicker (> 1.5 mm) or larger gel.

Contents : Approximately 0.2~0.4 mg/ml of each protein in buffer (20 mM Trisphosphate pH 7.5 at 25°C), 2% SDS, 1 mM 2-Mercaptoethanol, 3.6 M Urea, and 15% (v/v) Glycerol).


Quality Control : 5 μl of Trio-10 Prestained Protein Marker resolves 10 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.


Storage : 
Stable for up to 2 weeks at 25°C
Stable for up to 3 months at 4°C

For long term storage, store at -20°C 

PPM-180 Trio-10 Pre-stained marker 10 proteins 3 colors(10-180 kDa), 150 loads 500ul