Features ‧ Broad range: 10-180 kDa ‧ Ready-to-use: Supplied in a loading buffer for direct loading on gels ‧ Easy to identify: One green and one red band at 25 kDa and 75 kDa respectively ‧ Sharp bands Recommendations for Loading ‧ 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. ‧ 2 ~ 3 μl per well for general Western transferring. ‧ Apply more for thicker (> 1.5 mm) or larger gel.

Ordering Info

Storage : 
Stable for up to 2 weeks at 25°C
Stable for up to 3 months at 4°C

For long term storage, store at -20°C 

Contents : Approximately 0.2~0.4 mg/ml of each protein in buffer (20 mM Trisphosphate pH 7.5 at 25°C), 2% SDS, 1 mM 2-Mercaptoethanol, 3.6 M Urea, and 15% (v/v) Glycerol).

Quality Control : 5 μl of Trio-10 Prestained Protein Marker resolves 10 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.

Features ‧ Broad range: 6.5 - 270 kDa ‧ Ready-to-use: Supplied in a loading buffer for direct loading on gels ‧ Easy to identify: two orange bands at 30 kDa and 270 kDa and one green band at 52 kDa ‧ Sharp bands   Recommendations for Loading ‧ 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. ‧ 2.5 μl per well for general Western transferring.

Ordering Info

Quality Control : Under suggested conditions, Trio-10 Plus Prestained Protein Marker resolves 10 major bands in 4-12% Bis-Tris Gel (MES buffer) and after Western blotting to the nitrocellulose membrane.

Features ‧ Broad range: 3.5-245 kDa ‧ Ready-to-use: Supplied in a loading buffer for direct loading on gels ‧ Easy to identify: One green and one red band at 25 kDa and 75 kDa respectively ‧ Sharp bands Recommendations for Loading ‧ 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. ‧ 1.5~2.5 μl per well for general Western transferring. ‧ Apply more for thicker (> 1.5 mm) or larger gel.

Ordering Info

Storage : 

Stable for up to 2 weeks at 25°C
Stable for up to 3 months at 4°C
For long term storage, store at -20°C

Contents : Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 1 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).

Quality Control : Under suggested conditions, AvansBio Trio-13 Prestained Protein Marker resolves 13 major bands in polyacrylamide gel with appropriate buffers and after Western blotting to nitrocellulose membrane.

Features ‧ Broad range: 10-175 kDa ‧ Ready-to-use: supplied in a loading buffer for direct loading on gels ‧ Easy to identify: includes the ~10, ~40 and ~90 kDa reference bands coupled with an blue dye ‧ Sharp bands Recommendations for Loading 1. Thaw the Marker either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil. 2. Mix thoroughly to ensure the solution is homogeneous. 3. Load the following volumes of the Marker on SDS-polyacrylamide gel:   - 5 μl per well for mini-gels, 2.5 μl per well for blots   - 10 μl per well for large gels, 5 μl per well for blots

Ordering Info

Storage : 
Stable at 25°C for up to 2 weeks
Stable at +4°C for 3 months
Store at -20°C for 24 months

Contents : Approximately 0.2~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 1 mM Dithiothreitol, 4.8 M Urea, and 12 % (v/v) Glycerol).

Quality Control : Under suggested conditions, AvansBio Duo Prestained Protein Marker resolves 11 major bands in 15% SDS-PAGE (Tris-glycine buffer) and after Western blotting to nitrocellulose membrane.

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