Duo prestained protein marker

 

AvansBio Duo Prestained Protein Marker is a two-color protein standard with 11 pre-stained proteins covering a wide range molecular weights from 10 to 175 kDa. Proteins are covalently coupled with a pink chromophore except for three reference bands of blue color (at 10, 40, and 90 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). AvansBio Duo Prestained Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The Marker is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading. 


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Features

Broad range: 10-175 kDa
Ready-to-use: supplied in a loading buffer for direct loading on gels
Easy to identify: includes the ~10, ~40 and ~90 kDa reference
bands coupled with an blue dye
Sharp bands

 

Applications

Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis.
Monitoring of protein transfer onto membranes during Western blotting.
Sizing of proteins on SDS-polyacrylamide gels and Western blots.

 

 Recommendations for Loading

1. Thaw the Marker either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil.
2. Mix thoroughly to ensure the solution is homogeneous.
3. Load the following volumes of the Marker on SDS-polyacrylamide gel:
  - 5 μl per well for mini-gels, 2.5 μl per well for blots
  - 10 μl per well for large gels, 5 μl per well for blots

Contents : Approximately 0.2~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 1 mM Dithiothreitol, 4.8 M Urea, and 12 % (v/v) Glycerol).

 

Quality Control : Under suggested conditions, AvansBio Duo Prestained Protein Marker resolves 11 major bands in 15% SDS-PAGE (Tris-glycine buffer) and after Western blotting to nitrocellulose membrane.

 

Storage : 
Stable at 25°C for up to 2 weeks
Stable at +4°C for 3 months
Store at -20°C for 24 months

 

Part# DESCRIPTION Unit Size
PPM-175 Duo pre-stained marker 11 proteins 2 colors (10-175 kDa), 150 laods 500ul